What are the methods of blood cell counting?

Manual cell counting

• Counting chamber.
• Plating and CFU counting.
• Electrical resistance.
• Flow cytometry.
• Image analysis.
• Stereologic cell counting.
• Spectrophotometry.
• Impedance microbiology.

What will you use to dilute your blood sample for counting white blood cells?

Diluting the blood You can count blood cells with as little as a drop of blood. The dilution that is usually performed is 1:200 blood:isotonic solution. So for example, we could take 1μL of undiluted blood and add 199μL of isotonic solution (or do serial dilutions).

What are the diluents used for blood cells count?

Blood cell indices > White cell count The diluent contains an agent (glacial acetic acid) which lyses the red cells, allowing a proper count of white cells. Review the use of the hemocytometer and the use of the adjustable pipette.

Why is blood diluted 200 times for red cell count?

For RBC counting Thus, 1 portion out of 101 is discarded. So, 0.5 part of blood is in 100 parts of fluid or, 1 part of blood is mixed in 200 parts of fluid Thus, dilution factor for RBC counting is 200.

How do you calculate the dilution factor for cell counting?

Dilution Factor = Total Volume (Volume of sample + Volume of diluting liquid) / Volume of sample. Total viable cells/Sample = Viable Cells/ml x The original volume of fluid from which the cell sample was removed.

How do you manually count cells?

Manually Count Cells in Sample Focus both onto the grid pattern and the cell particles, and count the total number of cells found in 4 large corner squares. If cells are touching the 4 perimeter sides of a corner square, only count cells on 2 sides, either the 2 outer sides or 2 inner sides.

What is the dilution factor in the WBC pipette?

(Dilution factor is 20 for there is no mixing of cells till first 1 mark of the WBC pipette, hence 0.5 parts of blood are present in 10 parts of the diluting fluid-dilution factor, then, is 10/0.5 = 20).

How do you make diluted blood?

Blood is diluted 1 in 20 by taking 0.1 ml of blood into 1.9 ml of diluent (R3).

How will you get the 1/200 dilution in RBC count?

When blood is sucked up to 0.5 mark and the diluting fluid up to 101 marks, gives the 1:200 dilution of Blood: Diluting fluid and When the Blood is sucked up to 1 mark and the diluting fluid up to 101, gives the 1:100 dilution of Blood: Diluting fluid which is commonly used in anemic patients.

What is a 1 200 dilution?

200 (1/200 dilution) = the first step in the dilution factor; the second is 1/50, obtained as follows: 1 ml of serum + 199 ml of diluent = 1/200 dilution. 1 ml of 1/200 dilution + 49 ml of diluent = 1/50. To check: 50 × 200 = 10,000.

How do you calculate multiple dilutions?

In serial dilutions, you multiply the dilution factors for each step. The dilution factor or the dilution is the initial volume divided by the final volume. For example, if you add a 1 mL sample to 9 mL of diluent to get 10 mL of solution, DF=ViVf = 1mL10mL=110 .

What is dilution factor when doing WBC count?

What is dilution factor when one is doing WBC count? The standard dilution of blood for leukocyte counts is 1:20; therefore the dilution factor is 20. The volume of diluted blood used is based on the area and depth of the counting area. The area counted is 4 mm2 and the depth is 0.1 mm; therefore the volume factor is 0.4 mm3.

How do you dilute whole blood to count leukocytes?

Whole blood is diluted with a 3\% acetic acid solution, which hemolyzes mature erythrocytes and facilitates leukocyte counting. The standard dilution for leukocyte counts is 1:20. This dilution is prepared using the leukocyte Unopette system. The dilution is mixed well and incubated to permit lysis of the erythrocytes.

How do you calculate cell density from dilution factor?

We divide the cell density by the dilution factor and we get: 0.44 / 1.6 = 0.275 cells/mL. Double checking: 11 cells / 40mL = 0.275 cells/mL 🙂. When calculating in the same direction as the dilution, divide the cell density by the dilution factor. It makes sense as concentration decreases with higher volumes.

What is the volume of diluted blood in a blood count?

The volume of diluted blood used is based on the area and depth of the counting area. The area counted is 4 mm2 and the depth is 0.1 mm; therefore the volume factor is 0.4 mm3. Click to see full answer.